ABSTRACT
OBJECTIVE: The aim of this study was to assess the endothelium function in patients with Turner syndrome using videocapillaroscopy and to compare the results with healthy control. METHODS: Subjects and controls were studied in a temperature-controlled room, 20 days after no nailfold manipulations. The capillaries were visualized by a microscope connected to a television and a computer. The test of post-occlusive reactive hyperemia was performed using a sphygmomanometer attached to the fourth left finger, 20mmHg above maximum arterial pressure during 1 minute, and the following patterns were studied: area of transverse segment, maximal post-ischemia area and time to reach maximal post-ischemia area. RESULTS: The value of measure of transverse segment projected area , the maximal postischemia area of hand nailfold capillary loops using computerized videophotometry and the time to reach maximal post ischemia area were studied in 40 patients with Turner syndrome and 26 healthy women controls of comparable age (20±7.5 versus 18±8.1 years old; p=0.57). There were differences between transverse segment area (706.8±139.1 versus 548.8±117.2; p=0.001). Maximal post-ischemia area (891.3±226.1 versus 643.5±134.3; p=0.001) and the time to reach it (10.8±4.3 versus 5.5±2.5; p=0.001) were different between patients and controls. CONCLUSIONS: Changes of capillary response to ischemia could be observed in patients with Turner syndrome using videocapillaroscopy when they were compared to a healthy control group.
OBJETIVOS: O objetivo deste estudo foi avaliar a função endotelial de pacientes com síndrome de Turner, utilizando a videocapilaroscopia e comparar os resultados com grupo controle saudável. MÉTODOS: Pacientes e controles foram estudados em sala com temperatura controlada, após 20 dias, sem a manipulação das cutículas. Os capilares foram visualizados por microscópio conectado a televisão e computador. O teste de hiperemia reativa pós-oclusiva foi realizado utilizando-se esfigmomanômetro fixado no quarto quirodáctilo da mão esquerda, 20mmHg acima da pressão arterial máxima durante 1 minuto, e os seguintes parâmetros foram estudados: área do segmento transverso, área máxima pós-hiperemia e tempo para alcançar a área máxima pós-hiperemia. RESULTADOS: O valor da área do segmento transverso, área máxima pós-hiperemia dos capilares da região da mão usando-se a videocapilaroscopia computadorizada e o tempo para alcançar a área pós-hiperemia foram estudadas em 40 pacientes com síndrome de Turner e 26 controles do sexo feminino pareados para idade (20±7,5 versus 18±8,1 anos; p=0,57). Houve diferenças nos grupos quanto à área do segmento transverso (706,8±139,1 versus 548,8±117,2; p=0,001). A área máxima pós-hiperemia (891,3±226,1 versus 643,5±134,3; p=0,001) e o tempo para alcançá-la (10,8±4,3 versus 5,5±2,5; p=0,001) foram significativamente diferentes entre pacientes e controles. CONCLUSÃO: Houve alterações na resposta do capilar à isquemia em pacientes com síndrome de Turner utilizando-se videocapilaroscopia quando comparados ao grupo controle saudável.
Subject(s)
Humans , Male , Female , Adult , Microscopic Angioscopy/methods , Turner Syndrome/diagnosis , Control Groups , Endothelium/blood supplyABSTRACT
This paper reports the overall effects of three lectins, extracted from Canavalia brasiliensis, Dioclea violacea, and D. grandiflora, on BALB/c mice popliteal draining lymph nodes. These lectins have presented high stimulatory capacity on lymph node T cells. Additionally, they were able to induce apoptosis and inflammation (frequently associated with high endothelial venule necrosis). The data presented here suggest that the Diocleinae lectins studied can stimulate in vivo T cell activation and apoptosis, as well as present important side effects
Subject(s)
Animals , Mice , Male , Female , Apoptosis/drug effects , Fabaceae/chemistry , Lectins/pharmacology , Lymph Nodes/drug effects , Lymphocyte Activation/drug effects , Amino Acid Sequence , Cell Count , Endothelium/blood supply , Fabaceae/genetics , Inflammation/chemically induced , Lymph Nodes/pathology , Mice, Inbred BALB C , Necrosis , Receptors, Interleukin-2/metabolism , Venules/pathologyABSTRACT
To overcome the problems commonly involved in the culture of microvascular endothelial cells included unreliable isolation techniques and low cell-yields, a simplified protocol for the selective cultivation of rat heart microvascular endothelial cells [RHMEC], based on the cell specific expression of platelet-endothelial cell adhesion molecule PECAM-1 [CD31], was developed. Subconfluent primary cultures consisted of a mixture of endothelial cells, fibroblasts, and smooth muscle cells were isolated by their selective binding to magnetic beads coupled with anti-PECAM-I monoclonal antibody. After 2 immunomagnetic purification steps, a homogenous population of RHMEC was obtained, which showed typical cobblestone morphology, expressed CD3 1 and Von Willebr and factor proliferated in response to endothelial growth supplement and formed capillary-like tubes in a 3-dimensional ESH-gel matrix. This simple technique might facilitate a more widespread use of microvascular endothelial cell cultures obtained from different human or animal organs for functional in vitro studies